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KMID : 0616120110420010015
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Korean Journal of Pharmacognosy
2011 Volume.42 No. 1 p.15 ~ p.21
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Comparison of the Scrophulariae Radix of Gyeongbuk’s with that of Chinese: Content of Harpagoside and Inhibitory Effect of Nitric Oxide
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Zhang Hai-Yan
Kim Geum-Jin
Hwang In-Hyun
Cho Hi-Jae
Lee Seung-Ho
Na Min-Kyun
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Abstract
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Scrophulariae Radix (Scrophulariaceae) has been traditionally used to treat pharyngitis, laryngitis, tonsillitis, fever, and edema. To compare the quality of the Scrophulariae Radix produced from Gyeongbuk with that from China, a quantitative analysis on the major component, E-harpagoside, was performed by reversed-phase HPLC, and a relative inhibitory activity of nitric oxide (NO) was assayed in the Raw 264.7 cells. Using a Luna C18(2) column, we carried out quantitative analysis with a gradient of 0.1% phosphoric acid in water and acetonitrile. Our results demonstrated that the content of E-harpagoside in Gyeongbuk’s Scrophulariae Radix were three times higher than that of Chinese. Moreover, the inhibitory activity of Scrophulariae Radix of Gyeongbuk’s in the lipopolysaccharide (LPS)-induced NO production was higher than that of Chinese.
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KEYWORD
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Scrophulariae Radix, Scrophulariaceae, E-harpagoside, Quantitative analysis, Inhibition of nitric oxide
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